GenoFAB
Whole Plasmid Sequencing
Whole Plasmid Sequencing
High-end whole plasmid sequencing service for people who need to minimize the risk of sequencing errors. We prepare the plasmid solution, collect data using Nanopore and Illumina technologies, and perform the bioinformatics analysis.
Volume discounts: 10% for 24 plasmids. 20% for 96 plasmids.
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Plasmid sequencing process
Step 1: Place your order
Tell us how many plasmids you need to include in your order.
Step 2: Shipping
We will send you a box with a prepaid return label and prelabelled tubes to identify your plasmid solution. You can ship as little as 10 ng.
Ship your samples back to GenoFAB.
Step 3: Sample preparation
Upon receipt of your plasmids, we will transform them into bacteria, prepare glycerol stocks, and extract plasmid solutions. We record all these samples in your lab account.
Step 4: Sequencing
We sequence your plasmids with Nanopore and Illumina sequencers to collect two complementary data sets with different types of sequencing errors.
Step 5: Bioinformatics analysis and reporting.
We run the two datasets through our proprietary assembly pipeline to generate your plasmid physical sequence. Areas of uncertainty are confirmed by Sanger sequencing.